The Supplementary Material 1 file (not available in the manuscript) contains the following data:

1) The 214 references (literature papers) that were consulted for this research study.

2) Table S2: The initial raw data, which excludes the research gaps, showing CEC class, sampling mode, matrix, analytical method, etc.

3) Table S3: The initial raw data, which details the research gaps: high level class description, actual research gaps, and the relative frequency (number, percentage of total).

4) Table S4: Some typical classes of CECs.

5) Table S5: Some of the reported matrices analysed for CECs.

6) Table S6: Reported definition of a CEC and references.

7) Table S7: Summary of other reported descriptions (properties) of CECs.

8) Table S8: The International Organisation for Standardisation (ISO) requirements as per ISO ISO/IEC Directives Part 2 Principles and rules for the structure and drafting of ISO and IEC documents: Clauses on subdivisions of the document only.

The Supplementary Material 2 file contains Table S1: The initial raw data for the research gaps information only (Excel version).

Supplementary Material

Only two tables are provided in the manuscript:

Table 1: Summary of the reported CECs research gaps and the relative frequency.

Table 2: Quantitative chemical test method validation and key accreditation criteria comparison among some reputable international standard bodies.

From the literature review, a total of 214 papers were noted, with a total of 21 review articles (9.8%) (Supplementary Material 1). Of this total, a surprisingly high number were from South Africa alone: 117 (54.7%), just over 50%, of which 44 (20.6%) reports were associated with South Africa’s Water Research Commission (WRC) 2728.

The WRC was established in South Africa in terms of the Water Research Act (Act No 34 of 1971), following a period of serious water shortage. It was deemed to be of national importance to generate new knowledge and to promote the country’s water research purposefully, owing to the view held that water would be one of South Africa’s most limiting factors in the 21st century. Its vision is to have highly informed water decision-making through science and technology at all levels, in all stakeholder groups, and innovative water solutions through research and development, for South Africa, Africa and the world. Its Mission, Values and primary functions are available on its web site.

The common classes of CECs (Table S3) that were reported on, were 1) Chemical: pharmaceuticals, personal care products, steroids, chlorinated and brominated contaminants, PAHs, PCBs, phthalates, alkyl phenols, herbicides, organochlorine pesticides, engineered nanomaterials, etc., and

2) “Microbiological”: antibiotic resistance genes, human enteric bacteria and viruses, microbial pathogens (e.g., E Coli, rotavirus, Crypto, etc.), infectious biological water contaminants (e.g., E Coli isolates), cyanobacterial blooms (e.g., Microcystis), etc.

The sampling methods that was reported in the 214 references.(Table S1) was predominantly “grab”, with the following breakdown: Grab samples: ± 178 ( ± 82.4%), Composite samples: ± 11 ( ± 5.1%), Grab and Composite samples: ± 5 ( ± 2.3%), Polar Organic Chemical Integrative Samplers (POCIS): 1 ( ± 0.5%), and 24 hr flow proportional sampling: 1 ( ± 0.5%).

The reported matrices (Table S4) in the 214 studies, appeared to be quite extensive: from the most common, typical raw and treated drinking water, raw and treated wastewater, sludge, through to the less common ones: e.g., other solids, various biota and even human-based matrices.

The relative, approximate distribution was noted to be, in decreasing rank: 1) water (raw, source) (31%), 2) biota (26.6%), 3) waste water (13.3%), 4) food (11.4%), 5) solid (10.1%) and lastly, 6) air and human (7.6%).

These findings indicate and confirms that the CECs are rather widespread in the environment. We would also expect the most commonly tested matrix to be drinking (tap) water, which was ranked 1), at 31%. Surprisingly, the second most common matrix was not wastewater or similar, but rather the biota (26.6%).

The complexity of environmental matrices and the relatively low concentrations generally detected, require efficient, optimized sample pre-concentration-extraction techniques, and also, removal of any interferences that may be present before chromatographic analysis.

Whilst this aspect was not covered in this review, it should be briefly noted that Solid Phase Extraction (SPE) has been traditionally used for multi-residue analysis due to some advantages, such as preconcentration of the analytes 29. Despite the drawbacks of SPE, such as, (i) the use of large volumes of organic solvents in comparison with miniaturized techniques, (ii) it is time- consuming and (iii) high cost, it is still the most used technique due to the high enrichment factors and efficient reduction of interferences and matrix effects 303132. Other reported extraction techniques include: online SPE, solid phase micro extraction (SPME), multilayer SPE, fabric-phase sorptive extraction (FSPE) and liquid-liquid extraction, the latter being used for the analysis of volatile compounds 3334. The direct injection (DI) technique 35 is an alternative to reduce solvent consumption and analysis costs. Several authors have reported the use of specific ion exchange or graphitized carbon SPE, alone, or in tandem with HLB sorbent cartridges or pH adjustments prior to sample filtration-extraction 36. One technique that has not been extensively researched for the CECs application is QueChERS, which was originally applied to the multiresidue analysis of polar and nonpolar pesticide in fruit and vegetables 37. One recent QuEChERs approach was reported by Martinez-Piernas et al 38, for multiresidue- determination of 107 CECs in treated wastewater, using LC-quadrupole-linear ion trap tandem mass spectrometry.

This section will attempt to address some of the significant, common gaps identified in this study.

Method validation is defined as “the process of establishing the performance characteristics and limitations of a method and the identification of the influences which may change these characteristics and to what extent”.

Validation 454647 is the process that is followed to demonstrate with the provision of objective evidence, that a specified test method is suitable for the intended purpose. Performance characteristic “means functional quality that can be attributed to an analytical method”. Examples of typical performance characteristics 454647, for chemical test methods, include: selectivity, accuracy, trueness, recovery, precision, repeatability, reproducibility, limit of detection, limit of quantitation, ruggedness and stability. The differences due to matrices (“scope”) must be taken into account when testing different types of samples. The raw data should be evaluated with appropriate statistical methods.

Quality control and quality assurance includes interlaboratory comparisons, blind test samples analyzed by the laboratory and proficiency testing schemes 4852. Proficiency testing is an accreditation requirement for laboratories 4748, for the scope of accreditation (tests) being performed, and it should conform to the requirements of ISO 17043 51. It also includes internal quality control, which consists of all the procedures undertaken by a laboratory for the continuous evaluation of its testing work 454647. The main objective is to ensure the consistency of test results produced day-to-day and their conformity to defined criteria, or internal limits. A program of periodic checks is necessary to demonstrate that variability (e.g., between analysts and between equipment or materials, etc.) is under control.

One of the major CECs research gaps regarding the “Class (category) Number 2 (Table 1) “analytical tests” is the lack of comprehensive, standardized method validation guidelines, standardized tests, or standards. The availability of an internationally accepted standard for method validation will no doubt promote the development and availability of many more tests for CECs in various water, and other related, environmental matrices.

Many testing laboratories have achieved ISO/IEC 17025 accreditation for various chemical tests on water, and even on wastewater, soil, sludge, etc.

Accreditation is the confirmation by an accreditation body that the organization is competent to conduct its technical activities (tests or calibration) and to produce valid reliable results to specified requirements. Implementation of the requirements of a recognized standard as a management system gives confidence that the technical activities of an organization will consistently produce valid results. It is competence-based.

The ISO/IEC 17025:2017(E) document, titled General requirements for the competence of testing and calibration laboratories 47, is an international standard that has been developed with the objective of promoting confidence in the operation of testing and calibration laboratories. This standard covers both the technical and management system requirements that need to be fully met, for the accreditation of a test method; the generic requirements are summarized in Table 2. Testing laboratories that are ISO/IEC 17025-accredited are deemed competent to generate valid test results for their accredited test methods. In order for a test method to be ISO/IEC 17025-accredited, there is the prior need for method validation (technical), in addition to the laboratory complying with all the other technical and management requirements as per the ISO/IEC 17025 standard 47. The generic test method validation and ISO 17025 accreditation data requirements 4546, are summarized in Table 2.

For a country like South Africa, the South African National Accreditation System (SANAS) 53 is the single, national ISO/IEC 17025 Accreditation Body that gives formal recognition that Laboratories (e.g., testing, calibration), Certification Bodies, Inspection Bodies, Proficiency Testing Scheme Providers and Good Laboratory Practice (GLP) test facilities are competent to carry out specific tasks. SANAS is responsible for the accreditation of Certification bodies to ISO/IEC 17021, ISO/IEC 17024 and 65 (and the IAF interpretation thereof), and laboratories (testing and calibration) to ISO/IEC 17025.

Some of the key technical requirements, by some international scientific bodies, like the United States Environmental Protection Agency (US EPA) 54, the American Society for Testing and Materials (ASTM) 55, and the Association of Official Analytical Collaboration (AOAC) 56, compared with the requirements of the ISO/IEC 17025 guide, are summarized in Table 2.

The earlier version (2005), and this 2017 updated, current version document, was developed with the objective of promoting confidence in the operation of laboratories. It contains requirements (both management and technical) to enable them to demonstrate that they operate competently, and that they are able to generate valid test results. Use of this document will facilitate cooperation between laboratories and other bodies, will assist in the exchange of information and experience, and in the harmonization of standards and procedures. The acceptance of results between countries is facilitated if laboratories conform, by way of being accredited, to the standard.

The Clauses 4 to 8 are listed in Table 2. In addition to the international ISO 17025 guide, the South African national ISO 17025 accrediting body SANAS has further supplementary documents, for assisting and guiding laboratories (e.g., water testing) with meeting the accreditation requirements: e.g., for chemical tests: SANAS TR 26 03 and SANAS R 80-04.The corresponding supplementary SANAS requirements for validation and quality assurance in microbiological testing, are specifically outlined in the SANAS document TR 28-03 57.

The generic method validation requirements are fairly comprehensive. Some of the key performance characteristics, and others, recommended for validation, are: accuracy, trueness, bias, precision, selectivity, specificity, LOD, LOQ, ruggedness, range, linearity, calibration, sensitivity, and also Measurement Uncertainty (MU). However, there is no additional identification requirements specifically for chromatographic tests, using mass selective detectors, which is now very commonly used for the CECs screen and for their quantification.

A standard is an agreed, repeatable way of doing something. A published document contains a technical specification or other precise criteria designed to be used consistently as a rule, guideline, or definition. Standards help to make life simpler and to increase the reliability and the effectiveness of many goods and services we use. Standards are created by bringing together the experience and expertise of all interested parties, such as the producers, sellers, buyers, users and regulators of a particular material, product, process or service.

Various international guideline options, technical documents, etc., and even test methods, are already available. These include: 1) ISO/IEC 17025 47, 2) US EPA 54, 3) Eurachem 58, 4) SANTE 11312/2021 59, 5) ASTM 55, 6) AOAC 56 and 7) The Standard Methods For The Examination of Water and Wastewater textbook 60. Routine testing laboratories that are accredited to ISO/IEC 17025 standard, research institutes, academia, health and environmental protection institutes, etc., are already, in a very favorable position to submit their developed and validated chemical tests to ISO for standardization. It is hereby proposed that use is made of the current available guides.

These options, considered as possible candidates for proposed routes towards the standardization of chemical test methods for CECs, specifically by chromatography-mass spectrometry, will be briefly discussed and reviewed below. The criteria for each option are indicated by a "tick" (√). An overall comparison is summarized in Table 2.

i ) ISO/IEC 17025

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO- member bodies), including the South African Bureau of Standards (SABS) 61, South Africa's national standardization body.

ISO is an independent, non-governmental international organization with a membership of 167 national standards bodies. Through its members, it brings together experts to share knowledge and develop voluntary, consensus-based, market relevant International Standards, currently 24 599, that support innovation and provide solutions to global challenges. The standards cover almost all aspects of technology, management and manufacturing.

Electro technical standards are harmonized internationally by the International Organization for Standardization (ISO) and the International Electrotechnical Commission (IEC). The work of preparing International Standards is normally carried out through ISO technical committees, currently 809. In the field of conformity assessment, ISO and the IEC form the specialized system for standardization. Before submission of a new test method for approval to ISO, the country needs to approach their national ISO body.

ISO has a guidance document for submission of new work: ISO Guidance on new work, with 3 Parts, covering: Part 1 New standardization areas, Part 2: New fields of work – proposals for new committees, and Part 3: New work item proposals within existing committees. The relevant Form is: ISO FORM 1 PROPOSAL FOR A NEW FIELD OF TECHNICAL ACTIVITY, and the proposal must follow the guidelines as per the ISO document: ISO/IEC Directives, comprising two Parts. Part 1 is titled: Consolidated Supplement - Procedure for the technical work – procedures specific to ISO, 13^th Edition 2022 and the ISO/IEC Directives. Part 2 is titled: Principles and rules for the structure and drafting of ISO and IEC documents. This contains the principles and rules for the structure and drafting of International Standards, Technical Specifications and Publicly Available Specifications. As far as is practicable, these principles and rules also apply to Technical Reports and Guides. The ISO/IEC Directives, Part 2 (edition 9, 2021) Principles and rules for the structure and drafting of ISO and IEC documents (Table S8), states the general principles by which ISO and IEC documents are drafted and stipulates certain rules that shall always be applied in order to ensure that they are clear, precise and unambiguous. These rules are also important for ensuring that each document contributes effectively to the consistent and interdependent body of knowledge that ISO and IEC produce.

There is even a simple draft template for ISO standards. The purpose of this template is to simplify the drafting of International Standards and similar publications by providing a document "skeleton" that incorporates a range of predefined stylistic and structural rules, as well as ensuring that the electronic files of the document can be processed easily by the ISO Central Secretariat and other members of the ISO infrastructure. This aid to authoring is designed to save time and effort in the preparation and processing of standards by providing on-the-spot help concerning the drafting rules specified in the ISO/IEC Directives. There is even a model document available on the ISO web site, which demonstrates a simple application of the ISO/IEC Directives, Part 2, 2016, Principles and rules for the structure and drafting of ISO and IEC documents.

For standardization at a national level, e.g., for South Africa, the relevant authority is the South African Bureau of Standards (SABS) 61. As a founder member of the International Organization for Standardization (ISO), the SABS has built a reputation globally as a long-standing and widely respected role player in international standardization, and the leading standardization body in Africa.

In South Africa, the national process for the submission of a new test method, to ISO, is firstly via the SABS: a proposal is submitted, for the development of the standard in which a detailed justification for the need of the standard has been identified and how this will have an impact on the South African economy (SABS Project proposal and registration Form (AZ 96.22_2021/04/08 sabs pta)). The SABS is also a full participating member of the ISO technical committee on Water Quality, ISO/TC 147. The Technical Committee SABS/TC 147/SC 02 deals with “Water - Physical, chemical and biochemical methods”.

 ii) The United States Environmental Protection Agency (US EPA) (2016): Validation and Peer Review of U.S. Environmental Protection Agency Chemical Methods of Analysis

The US EPA program offices publish a wide variety of measurement methods for use by EPA personnel, other government agencies, and the private sector. These methods may originate from many sources, such as the EPA laboratories, EPA contractors, scientific organizations, other government laboratories, and from the private sector. Since these methods may be published as regulations, incorporated by reference in regulations, or published as guidance, they must be thoroughly tested and peer reviewed prior to publication as US EPA methods.

This document provides Agency-wide guidance for EPA personnel who will evaluate the performance and suitability of new chemical methods of analysis before EPA publication. The method validation principles contained herein are based on current, international approaches and guidelines for intralaboratory (single laboratory) and interlaboratory (multiple laboratory) method validation studies. This guide is referenced to some documents by the following relevant, international bodies: Eurachem 1998, IUPAC 2002, ISO 5725-1 1994, ISO (VIM) 1993, ASTM 2003, and AOAC International 2002.

Peer review is required for the EPA chemical methods of analysis. The EPA Science Policy Council Peer Review Handbook provides Agency-wide requirements and options for that process.

The key performance characteristics that are covered are: accuracy, trueness, bias, selectivity, LOD, LOQ, ruggedness, precision (repeatability and reproducibility), sensitivity, calibration, linearity, range, analyte stability, sample stability. Whilst the Measurement Uncertainty is defined at the end of the guide, it appears not to be a mandatory requirement. Interlaboratory studies determine whether an analytical method can be transferred for use in other laboratories and whether it can be used for regulatory testing. A method that proved rugged for use in one laboratory may lose that characteristic when tried in several other laboratories. This guide does state that an interlaboratory method validation study should be completed prior to publishing an EPA method for general use; it is an expected topic area of the method validation summary (report). The extent of the study will vary, depending on available resources and the intended use and applicability of the method. An interlaboratory method validation study is a practical testing of the written method on identical test materials (samples), usually derived from split samples, by a number of laboratories. The study is not intended to evaluate laboratories; it is intended to evaluate method reproducibility among laboratories.

Here again, there is no specific, additional identification requirements for chromatographic tests, with mass selective detectors, now commonly used for CECs screen, identification and quantification.

iii) The US EPA Alternate Test Procedure (ATP)

The Clean Water Act Alternate Test Procedure (ATP) program is described at 40 CFR 136.4 and 136.5. This program provides a mechanism for the submission and review of an application for nationwide use or limited use of an ATP for measurement of a pollutant as an alternative to the methods approved at 40 CFR Part 136. An ATP may fall into one of two categories: 1) A method using a determinative technique (e.g., a pollutant detector) different from that in an existing Part 136 method (for method validation and evaluation purposes, this type of method is referred to as a new method), or 2) A modification to a Part 136 method that falls outside the scope of the modification flexibility described in the Part 136 method, or at 40 CFR 136.6 (for validation and evaluation purposes, this type of method is referred to as an ATP). A method developer may apply for review of a method modification or a new method through the ATP program.

The EPA has provided detailed guidance about the kind of information it needs to evaluate methods for potential approval. The Agency conducts a notice-and-comment rulemaking process for methods evaluated under the ATP program, before they are incorporated into the Part 136 regulations. The types of information required for an application are described in three protocol documents: two for chemical methods, and one for microbiological methods.

EPA finalized the protocols in 2018 for the review of ATPs and new methods for chemical analytes, based on public comments submitted on the 2016 draft protocols: Protocols for EPA Review of Alternate Test Procedures (February 2018).

Before submitting a method to the US EPA for an ATP review, a developer confers with the EPA to design a method validation study. This study tests the new or modified method in several representative matrices and independent laboratories. The method must also be written in a standard format that includes all of the steps in an approved method, such as sample and data handling, and quality assurance requirements.

The US EPA has 4 guide documents related to its Clean Water Act, the CWA Alternate Test Program: for test methods, namely:

1) Protocol for Review and Validation of Alternate Test Procedures for Regulated Organic and Inorganic Analytes in Wastewater Under EPA’s Alternate Test Procedure Program (pdf) (807.67 KB, February 2018, 821-B-18-002). The protocol provides guidance for validation, submission, and EPA review of ATP applications under EPA’s National ATP Program submitted for modifications of an EPA-approved method or a procedure that uses the same determinative technique and measures the same analyte(s) of interest as an approved method.

2) Protocol for Review and Validation of New Methods for Regulated Organic and Inorganic Analytes in Wastewater Under EPA’s Alternate Test Procedure Program (pdf) (854.53 KB, February 2018, 821-B-18-001). This protocol represents EPA’s “best thinking” about the information that is useful in making the determination of whether or not to approve use of any new method for organic and inorganic analytes.

3) EPA Microbiological Alternate Test Procedure (ATP) Protocol for Drinking Water, Ambient Water, Wastewater and Sewage Sludge Monitoring Methods (pdf) (830.84 KB, September 2010, 821-B-10-001) and

4) Guidelines and Format for Methods to be Proposed at 40 CFR Part 136 or Part 141 (1996).

The EPA has already developed 12 test methods by GC- and LC-MS, covering pharmaceuticals (Method 542), pesticides (Method 1699) and even per-and polyfluoroalkyl substances (Method 533, 537.1), in various matrices (water, soil, sediment, bio solid).

iv) Eurachem (2014) The Fitness for Purpose of Analytical Methods A Laboratory Guide to Method Validation and Related Topics and Planning and Reporting Method Validation Studies (2019) Supplement to Eurachem Guide on the Fitness for Purpose of Analytical Methods

This Guide, like the first Edition (1998) is based on, and is consistent with, the principles of ISO 17025. It further cross-references other relevant international guide documents, like ISO 9001 2008, VIM 2012, AOAC 2002, ASTSM 2012, IUPAC 2001, USP 2003, IUPAC 1995, and the Council Directive EC 2009 and Commission Decision EC 2002.

An initiative in the UK to promote good practice in analytical measurement has identified six principles of analytical practice which, taken together, are considered to constitute best practice. This document is principally intended to assist laboratories in implementing Principle 2, by giving guidance on the evaluation of testing methods to show that they are “fit-for-purpose”. The additional 2019 Supplement serves as guidance for, specifically, the planning and reporting of validation studies. This supplementary document is not intended to be used in isolation; it should be used in conjunction with the Guide. The aim is to provide a clear plan for the entire validation study, covering the performance characteristics that will be studied, the target value for each performance characteristic, the materials that will be analyzed, the level of replication and order of the experiments, any statistical analysis that will be used, and how the method will be judged as being “fit- for- purpose”.

It includes the typical performance characteristics: bias, selectivity, LOD, LOQ, ruggedness, precision (repeatability, intermediate, reproducibility), sensitivity, range, analytic stability, and MU.

Here again, this guide has no specific, additional identification requirements for chromatographic tests, with mass selective detectors, now commonly used for CECs screen, identification and quantification.

v) SANTE (2021)

This document 59 is intended for laboratories involved in the official control of pesticide residues in food and feed across the European Union (EU). It describes the method validation and analytical quality control (AQC) requirements to support the validity of data reported within the framework of official controls on pesticide residues, including monitoring data sent to the European Food Safety Authority (EFSA), and is used for checking compliance with maximum residue levels (MRLs), enforcement actions, or the assessment of consumer exposure. This document is more importantly, complementary and integral to the requirements specified in the ISO/IEC 17025 guide.

It specifies that within-laboratory method validation should be performed to provide evidence that a method is fit for the intended purpose. As per this guide, method validation is a requirement of accreditation bodies, and must be supported and extended by method performance verification during routine analysis (analytical quality control and on-going method validation). Typically, with each batch of samples routinely analyzed, one or more samples of different commodities from the applicable commodity category are spiked with the analytes and analyzed concurrently with the samples.

Sample extracts are normally analyzed using capillary gas chromatography (GC) and/or high performance or ultra-performance liquid chromatography (HPLC or UPLC) coupled to mass spectrometry (MS) for the identification and quantification of pesticides in food and feed samples. There are options for the use of various MS detection systems. For unit mass resolution, the typical systems listed are single MS (quad, ion trap, TOF) and MS/MS (triple quadrupole, ion trap, Q-trap, Q-TOF, Q-Orbitrap). For accurate mass measurement resolution, the corresponding typical systems listed are High resolution MS ((Q-)TOF, (Q-)Orbitrap). The Acquisition modes are listed for each mass resolution option. There are also specific requirements for identification for each mass resolution type used.

Typical ionization techniques are electron ionization (EI), chemical ionization (CI), atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI). Different acquisition modes may be used, such as full-scan, selected ion monitoring (SIM), selected reaction monitoring (SRM) and multiple reaction monitoring (MRM).

The guide specifies the following generic method validation parameters, with criteria: sensitivity (linearity), matrix effect, LOQ, specificity, recovery, precision (repeatability RSDr and within-lab reproducibility RSDwR) and robustness, ion ratio and retention time.

Besides quantitative validation aspects, the guide also specifies the identification parameters that must be assessed for MS-based methods: 1) Specificity, 2) Ion ratio, 2) Retention time, 3) Matrix effect. Of note is a specific section titled “Identification of analytes and the confirmation of results”, covering the following sub-categories: Mass spectrometry coupled to chromatography, Requirements for chromatography, Requirements for mass spectrometry (MS), Recommendations regarding identification using MS spectra, and the Requirements for identification using selected ions, for different MS techniques (single quad MS, triple quad MS/MS, high resolution MS). There is also a specific section for the “Confirmation of results”, and for “Reporting results” (expression, calculation, correction for method bias, rounding off data, and Qualifying results with ).

vi) Miscellaneous Journal publication 2020 (40): Wide-scope target screening of>2000 emerging contaminants in wastewater samples with UPLC-Q- ToF -HRMS/MS and smart evaluation of its performance through the validation of 195 selected representative analytes

The determination of the CECs in environmental samples constitutes a great challenge. The most common choice has been the use of multiresidue methods that include a limited number of compounds (normally < 100). This automatically reveals a gap in environmental analysis concerning methods and techniques that can analyze simultaneously, a higher number of CECs. Advances in the high resolving power mass analyzers (HRMS) have contributed towards the development of real wide-scope multi-residue screening methods, that also offer the potential of retrieving information about new analytes in post-acquisition approaches (retrospective analysis).

To date, one of the main deficiencies in wide-scope target screening analysis by HRMS methods is the lack of standardized criteria and harmonized guidance for the accurate identification and quantitation of the analytes. For a comprehensive target analysis the use of reference standards is necessary in order to compare (i) retention times (RTs), (ii) MS spectra profiles (precursor ion, adducts and in-source fragments) and (iii) MS/MS spectra (fragment ions and ratios). To reduce the number of false negative and false positive findings, a careful optimization of the data processing parameters (mass accuracy, RT or signal thresholds) is essential. Since the size of HRMS data is enormous, automated solutions are required. Regarding the identification and quantitation, the resolving power of the mass analyzer is of great significance. The criteria defined in the 1) Commission Decision 2002/657/EC (EC, 2002) (62), and in 2) SANCO 12571/2013 (SANCO, 2013) (63) and the previous version of SANTE 11813/2017(SANTE, 2017) (64) do not take full advantage of the available instrumentation capabilities and are still more low-resolution oriented. In addition, the compounds used as a validation dataset should be selected upon well-defined criteria and follow a uniform protocol.

This work (40) references the deficiencies in these guides: in addition to the specified Retention time, it has the following additional criteria for MS-based methods regarding “Identification”: 1) Decision Limit, 2) Detection capability, 3) Mass accuracy, 4) Isotope fit ,5) Fragmentation.

vii) ASTM

More than 30,000 people from 150 countries create and update standards through ASTM

International, one of the world’s most respected standards development organizations. The high quality of ASTM International standards is driven by the expertise and judgment of its members who represent industry, governments, academia, trade groups, consumers, and others. Their contributions are why ASTM International standards are known for high quality and market relevance across many industries.

The Types of Standards are the following: 1) test method, 2) specification, 3) guide, 4) practice, 5) classification, and 6) terminology.

Over 50 Proficiency Testing programs help laboratories evaluate, improve, and document their performance in conducting test methods when compared to other laboratories. More than 5,000 participants worldwide are involved in these statistical quality assurance programs that help laboratories to meet accreditation requirements.

The Memorandum of Understanding program promotes: 1) communication among standards bodies, 2) awareness of standardization systems, 3) development of national standards, 4) reduced duplication of efforts, and 5) broader economic development efforts.

 The process used by ASTM to develop standards is extremely flexible, honed over 111 years to accommodate a diverse collection of activities. Test methods, specifications, classifications, practices, guides, and terminology are different categories of standards offered by ASTM. Areas ranging from petroleum, steel, and plastics to homeland security, unmanned vehicles, and sustainability have all achieved standards-based solutions via ASTM's process.

 ASTM receives a variety of requests for new standards development activities, ranging from a single standard to a new main technical committee. It is important to note that not all requests ultimately reach fruition. As the organizational process evolves, it may be determined that the stakeholder interest is insufficient, other standards may exist that satisfy the particular need, or that it is premature for a consensus standards program. When a request is initially submitted, ASTM maps the scope and subject area to the existing committee population. If they are able to find an appropriate venue, they coordinate with the officers of the committee and subcommittee(s) in question. If the request covers an area unrepresented within ASTM, they proceed with their new activity organizational process.

ASTM has the guide titled: Form and style for ASTM Standards (September 2022).

This manual is the basic textbook for anyone writing an ASTM standard. A study of Parts A, B, C, or E will show the proper form for the principal types of standards, including a detailed explanation of how to write each section, from the title to the appendices.

If one is drafting a new ASTM standard (Test Method, Specification, Guide/Practice, Classification, or Terminology) the pre-formatted MS Word templates that will help to speed up the process, are available. These templates insert all of the required form and style elements as specified in The Form and Style for ASTM Standards manual, also known as "The Blue Book". They are built on the existing functionality of MS Word 97/2000; one can alter them to suit individual needs.

viii) AOAC

The Legal name is: AOAC INTERNATIONAL, and the Long-form name is: ASSOCIATION OF OFFICIAL ANALYTICAL COLLABORATION (AOAC) INTERNATIONAL. AOAC INTERNATIONAL is a 501(c) 3, independent, third party, not-for-profit association and voluntary consensus standards developing organization.

AOAC INTERNATIONAL brings together government, industry, and academia to establish standard methods of analysis that ensure the safety and integrity of foods and other products (dietary supplements, pharmaceuticals) that impact public health around the world. As a leader of analytical excellence, AOAC INTERNATIONAL advances food safety, food integrity, and public health, by bringing together members, organizations, and experts dedicated to developing and validating standards, methods, and technologies of global relevance.

Their key initiatives are:

1) Official Methods of Analysis Program: is the AOAC INTERNATIONAL’s premier methods program. Approved methods undergo rigorous, systematic, scientific scrutiny to ensure they are highly credible, defensible and that can be used with confidence by industry, regulatory agencies, research organizations, testing laboratories, and academic institutions.

2) AOAC Research Institute: is a division of AOAC INTERNATIONAL that promotes and conduct activities to help develop, improve, and validate proprietary testing methods and products.

3) Proficiency Testing (PT): helps laboratories to compete in the global marketplace by demonstrating and certifying that they meet the highest international standards for accuracy, reliability, and compliance.

The Appendix F: Guidelines for Standard Method Performance Requirements (SMPR) (AOAC, 2016) are intended to provide basic information for working groups assigned to prepare SMPRs. The guidelines consist of the standard format of an SMPR, followed by a series of informative tables and annexes.

Information about method requirements is itemized into nine categories: (1) intended use, 2) applicability, 3) analytical technique, 4) definitions, 5) method performance requirements, 6) system suitability, 7) reference materials, 8) validation guidance, and 9) maximum time-to-determination.

For a Quantitative method (trace or contaminant (Single Lab Validation)), the prescribed generic guidelines for standard method performance requirements are: Applicable Range, Bias, Precision, Recovery, LOQ, Reproducibility: RSD_R or Target Measurement Uncertainty.

ix) Standard Methods for the Examination of Water & Wastewater textbook (Standard Methods)

This comprehensive reference covers all aspects of water and wastewater analysis techniques. Standard Methods is a joint publication of the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF). The work of the Standard Methods committees of APHA, AWWH and WEF is coordinated by a Joint Editorial Board (JEB), on which all three are represented.

For each new edition, both the technical criteria for selection and the formal procedures for their approval are reviewed critically. With regard to approval procedure, standard methods presented have been peer reviewed and are supported by the largest number of qualified people. In this way, Standard Methods represent a true consensus of expert opinion.

The methods presented in Standard Methods are believed to be the best available, and broadly accepted procedures for analyzing (physical, chemical, microscopic, bacteriological) water, wastewaters and related materials. They represent the recommendations of specialists, ratified by a large number of analysts, and others of more general expertise. As such, they are truly consensus standards, which offer a valid and recognized basis for control and evaluation.

A Joint Task Group (JTG) is established for each section; appointment of an individual to the Team is based on their recognized expertise and interest. The Group reviews the pertinent methods, or methods from the previous edition, reviews current methodology in the literature, evaluate new methods relevant to a particular Section, and address any specific issues of concern received by the Standard Methods Committee. Once a Joint Task Group has finished with and approved the work on its specific Section, the manuscript is edited and submitted to the relevant Standard Methods Committee members who review and vote on Sections in a given Part. The JEB makes a final decision on how an issue is to be handled.

The technical criteria for selecting methods are applied by the Joint Task Groups and the individuals reviewing their recommendations; the JEB only provides general guidelines. In addition to the classical concepts of precision, bias and Minimum Detectable Concentration, test method selection also must consider other issues like: the time required to obtain a result, specialized equipment and analyst training needs, cost of analysis and the feasibility of its widespread use.

Methods published in Standard Methods are divided into two fundamental classes: Proposed and Standard. Regardless of class, all methods must be approved by the Standard Methods Committee:

1 Proposed: this method must undergo development and validation that meets the requirements of Section 1040A of Standard Methods.

2 Standard: a procedure qualifies as a Standard Method in one of two ways: a) the procedure has undergone development, validation and collaborative testing that meet the requirements set forth in Section 1040B and C of Standard Methods, and it is “widely used” or b) the procedure is “widely used” and it has appeared in Standard Methods for at least 5 years.

When an entirely new method is developed by accepted research procedures or an existing method is modified to meet special requirements, method validation by a 3-step process is required:

1) Determination of single operator characteristics: precision and bias: requires determining detection and reporting levels, analytical range, method bias (its systematic error), matrix effects, and interferences.

2) Analysis of independently prepared unknown samples: requires the analysis of independently prepared standards, by the standard operating procedure for the test method, whose value is unknown to the analyst. Unknown samples are obtained from other personnel in the laboratory, using either purchased analytical-grade reagents, or standards traceable to the National Institute of Standards Technology (NIST). Performance evaluation samples, if available, from accredited proficiency test (PT) providers (schemes) are also recommended.

3) Determination of method ruggedness: is the stability of the result produced when steps in the method are varied. It is especially important to determine this characteristic if the method will be proposed as a standard or reference method.

4) Equivalency testing: after a new method has been validated by the above procedures, it may be necessary to test it for equivalency to the standard method, unless they do not exist. Statistical steps with references are further provided in the textbook.

5) Collaborative multi-laboratory testing: After a new method has been developed and validated, it is then determined whether it should be made a “Standard method”, via the Collaborative Multilaboratory test. Here multiple laboratories collaborate by using the same SOP to analyse a select number of samples to determine the method’s bias and precision. Factors to be considered are a precisely written SOP, the number of variables to be tested (laboratories, apparatus, operators, levels (concentration)), the number of levels to be tested, the number of replicates required, and possibly matrix effects.

Table 2 gives a snapshot summary of some of the main criteria prescribed by the well-known, international technical bodies in the field of analytical chemical test method development-validation.

It appears that the technical requirements specified in the ISO/IEC, and the SANTE guides (followed by the US EPA/Eurachem) are fairly extensive, compared to that in the ASTM/AOAC/Standard Methods. Whilst all guides address the minimum generic method validation/performance requirements/criteria fairly well, the additional “Identification for MS-based methods” requirements are only prescribed by the SANTE guide and by the recent work by Gago-Ferrero et al 40.

It is proposed that researchers working towards the development-validation of standardized chemical test methods by chromatography-mass spectrometry can use any combination of these guides, in a complementary manner, preferably the SANTE and the ISO/IEC 17025, and also address the additional MS requirements, as described by Gago-Ferrero et al 40, during the test method development-validation.

The following are, inter alia, deserving of similar attention, that may have been partially covered here, and warrant further research:

1) Alternative descriptions for the definition of a CEC: This was partially addressed in this study (Table S6).

2) Risk assessment/toxicity testing: the reader can consult various reported references 65.

3) The management of CECs: This is another broad area that covers: reduction, removal, regulation, education, advanced treatment options, etc.

4) Target/Contaminant List: The US EPA has, for example, a Contaminant Candidate List 5 (CCL 5) 66. CCL 5 includes 66 chemicals, 3 chemical groups (cyanotoxins, disinfection byproducts (DBPs), and per- and polyfluoroalkyl substances (PFAS)), and 12 microbial contaminants. It is proposed that researchers can begin with a proposed list, at national level, based on potential sources.

For a potential target list of priority CECs to quantify, or screen for, a good starting point would be the potential source/s of the contaminants that needs to be first identified. For example, with the HIV and Tuberculosis (TB) disease burden in South Africa, it can be expected that relatively more related treatment drugs, like the anti-retro virals and anti-TB drugs/pharmaceuticals, will be found in raw source water, raw or treated wastewater, and in drinking water.

5) Complementary use of 2-dimensional (2-D) gas chromatography (GCxGC) 67: Test methods can be a combination of targeted analysis, like tandem mass spectrometry, and non-target screen, using 2-dimensional GCXGC-Q-TOF-MS, LC-QTOF-MS. Some of the major advantages of 2D-GCXGC, coupled to a mass spectrometric detector, include the following:

i) The chromatographic separation space is significantly increased compared to a standard 1D-GC analysis, yielding a big leap in potential peak capacity.

ii) Besides increased resolution, the resulting chromatogram is also structured so that similar compounds elute in clustered bands throughout the chromatogram plane.

iii) The clustering of structurally and chemically similar compounds offers a helpful clue that can help in peak identification.

iv) Increased compound detectability, or signal-to-noise ratio (S/N) (sensitivity).

v) Saves time with Multi-Class compound analyses possible in a single analysis.

vi) Reduced solvent usage with simplified sample preparation.

vii) Decreased manual labor with “Automated Peak Identification”.

With a non-specific detector like the ECD or FID, the contour plot can be used for grouping compounds based on their chemical class. When a mass spectrometer (MS) is used as a detector, unknown peaks can be further identified by searching a mass spectral library (for example, NIST).

viii) Automated identification of unknowns (analyte peaks) with time reduction.

ix) Accelerate discovery with increased peak capacity.

x) Savings on costs with the use of economical detectors.

Increasing the chromatographic resolution with GCxGC reduces the need for expensive mass spectrometer detectors; the FID is a robust, economical, and simple to operate detector.

xi) Viability in a routine laboratory setting.

Saving time in sample preparation, instrumental analysis, and non-target data review can be an immediate payoff when a GCxGC system is implemented. The ability to analyze an extensive range of complex samples with the simultaneous target and non-target detection is an attractive prospect that can be leveraged in a competitive landscape.

6) An international Data Base for CECs.: The possible matrix elements could include: already published research, technical application notes from analytical equipment vendors, validated or partially validated test methods, “hot spots” and identified CECs at a national level, etc.

7) Chlorine-resistant pathogens (bacteria) in disinfected drinking water 68:

Umgeni Water has at least one project addressing this area in the 5-year R&D Master Plan.

8) Per- and poly-fluoro alkyl substances, the “forever chemicals: PFAS (PFOS and PFOA). Whilst this review identified 19 research papers, there has been subsequently a tremendous increase in publications, covering their source, occurrence, toxic effects 69, analytical techniques for their determination 70, their degradation 71, their removal 72, etc.

The U.S EPA SW-846 Test Method 8327: Per-and Polyfluoroalkyl Substances (PFAS) by Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) (73) is now standardized for a selected 24 PFAS contaminants in non-potable water. The method was validated with real water samples testing, in a multilaboratory (eight) study, at 200 ng/L and at 60 ng/L for recovery (range = 75-130% at 60 ng/L) and precision.

There is additionally at least two WRC-funded PFAS monitoring-related projects in South Africa; one study has just been completed and the relevant research reports (Project No. 2019/2020-00187) will be available 28 once they are finalized.

Human exposure to PFAS alternatives and emerging PFAS, which were proposed as substitutes for legacy PFAS is unknown. Short-chain PFSA and PFCA homologues, like PFBS and PFHxA, are just as persistent as legacy and historical PFAS in the environment. There are limited data and information available on the occurrence, sources and fate of emerging PFAS in the environment than there is for the legacy PFAS. It is necessary to create a complete and trustworthy inventory of alternatives for legacy PFAS.

Some recently reported gaps include their potentially large number present in the environment that is yet to be identified, the unknown extent of their environmental pollution, further research to improve our understanding of their behavior and hazards they pose to the environment and human health, and whether they can be degraded or metabolized by humans in environmentally relevant conditions.

Between the time of this study inception (2019) and the present, various other publications have appeared on this, and related topics, that are not addressed here. Various other pertinent publications have also not been referenced here due to journal space requirements.